Since June of this year there has been a spate of yellowing and senescing (dying) of leaves of certain raspberries.  This problem is widespread in the Watsonville and Salinas production district and first appears as a yellowing of the older leaves toward the bottom of the plant.  The symptoms appear evenly distributed through the field with very little patchiness in incidence.  The yellowing tends to follow a mottled pattern (Photo 2 below) and surprisingly can be pretty bounded on the leaf, for example on one half and not the other.  Affected leaves end up separating from the main plant quite easily.  The yellowing and dieback most often occurs on the lower half of the plant, and rarely advances higher nor does it result in total plant death.  From growers and personal observations this yellowing and senescing usually does not appear to result in any reduction in plant vigor or yield, but there are occasions of total plant loss.  There are reports of occasional loss of fruit integrity and crumbliness in affected fields, but this is strictly anecdotal.

The lengthy report below is a summary of the multifold approach we took to studying this issue followed by a discussion at the end regarding what is the most likely cause.

Arthropods:  Arthropods are not the cause of this problem.  One grower reported a correlation with a certain type of mite and while there were patches of twospotted spider mites in some locations, in others there were none nor had there ever been.  Beyond the mites, no other arthropods capable of causing leaf discoloration or necrosis were found.

Diseases:   The leaf yellowing is not caused by a pathogen.  A total of five whole plant samples (plant tops and roots) from several fields experiencing leaf yellowing were submitted to the UCCE diagnostic laboratory in Salinas, and in only one case did the test come up positive for a species of Phytophthora.  As leaf yellowing can be caused by virus infection, for example Raspberry Bushy Dwarf Virus on the Autumn series of red raspberry, a set of leaf samples was tested at the UCCE diagnostic lab in Salinas and another distinct set totaling five samples was submitted to the CDFA disease diagnostic laboratory in Sacramento.  All tests for virus came back completely negative.

Nutritional:  Levels of nitrogen and phosphorous are below sufficiency and substantially lower in yellow leaves than in green leaves, and levels of calcium are above sufficiency (and well above sufficiency in a field of healthy green plants) and higher in yellow leaves than in green leaves.  Soil concentrations of nitrates are below recommended levels of at least 10 ppm in three of four fields experiencing yellow leaves.  Concentrations of the salts sodium and chloride are substantially lower in all fields experiencing leaf yellowing than a field of symptomless, apparently healthy plants.

Table 1.  Leaf Samples. ‘Healthy Green’ refers to leaf samples taken from a field experiencing no yellowing anywhere; other Green and Yellow are paired sets coming from the same field and leaves collected from floricane at the same approximate height of about 18" above the soil.  Samples consisted of at least 15 leaves coming from various parts of the field taken between July 16 and July 27.

^*Healthy sample excluded from these calculations.

Table 2. Soil Samples. Soil samples are a total of 10 6” deep cores taken from areas experiencing yellow leaves.  The surface crust of the soil was brushed away before sampling.   All soil samples were taken July 27.

^*The designations “Healthy Green, Yellow 1, Yellow 2 and so on correspond with the same designations for the leaves above, meaning they come from the same fields.

Discussion:  This issue of yellowing leaves in raspberry is not being caused by insects, mites or disease but we do find a striking difference in the concentration of some nutrients in affected leaves and the soils from from which they come.  Since all the fields in question here are being managed by extremely competent and experienced growers we can be certain this nutritional deficiency is not being caused by a simple lack of fertilizer application, so we must explore this a little further.

All of the plants experiencing leaf yellowing and senescence are being grown in macro-tunnels.  The PCA attending each of these fields reports that they have been very warm on the inside compared to outside and interestingly found a high level of humidity as well.   This adds something to our investigation, since high temperatures can be a factor affecting the growth of plants, especially plants such as raspberry adapted to temperate climates. Heat injury in plants often results leaf yellowing and senescence.

Leaf nitrogen and phosphorous are lower in yellow leaves than green leaves.  Leaf senescence is often preceded by the degradation and subsequent loss of chlorophyll, the lack of which of course means the leaf stops being green.  A quick perusal of a book concerned with the mineral nutrition of plants (the excellent “Mineral Nutrition of Plants” by Horst Marschner I recommend highly) tells us that leaf senescence results in a net mobilization or export of nutrients from the dying tissue to still living and growing tissue.   And we are seeing exactly this in the fields, since the plant tops continue to flourish and produce flowers and fruit all the while the bottoms are yellowing and dying.

But what of the higher concentrations of calcium in yellow leaves compared to green?  They are quite high, near or above what are normally considered levels of sufficiency.  Calcium, in contrast with other plant essential minerals is moved from the roots to the rest of the plant via evapotranspiration through the water conducting elements (also known as the xylem).  This is meaningful since this means we tend to see higher levels of calcium deposition in plants moving lots of water.

Soil concentrations of nitrate, sodium and chloride are all low in fields experiencing leaf yellowing and necrosis.  Knowing however that all three are leachable by water is again helpful.

The Bottom Line: What we are getting is that the tunnels are hot and killing some of the leaves towards the bottom of the plant.  The plants continue to grow though, reallocating nutrients from the dying leaves to the younger ones and pulling up plenty of water because the rate of evapotranspiration is high in the heat of the tunnel.  The grower response of adding more water to keep up is of course the correct one, but this is resulting in a lot of leaching.  For salts like sodium and chloride this good, for a plant essential ion like nitrate it is not.  So not only is nitrogen being transported away from the dying leaves, it is also being leached away in the soil before the plant can get it.

Probably the best response to this situation would be the one which has already been taken, and that is to open the tunnels to enhance air circulation and lower the internal temperatures. 

I would like to thank PCA Eryn Gray for sharing his data and insight along with the growers involved in this work without whose participation none of this would have possible.

I had a brief chat concerning albino strawberry fruit this morning.  Maybe it shouldn't be surprising that I get a call concerning this disorder since it has been rather cool and cloudy for close to a week.

Anyway, as one can see from the picture of some albino fruit off of Diamante strawberry (it's from a few years ago), the fruit takes on a pale whitish color,  with just a bit of red around each achene.  The lack of ripe red color comes from an inadequate supply of sugar during maturation, and indeed such fruit are insipid and tasteless. 

On the Central Coast, strawberry fruit albinism tends to come in fields experiencing heavy nitrogen fertility or really frequent irrigation during an overcast period on the heels of a spell of sunny and fairly warm weather.   The symptoms might be exacerbated in the shadier parts of the plant canopy, and the literature says it shows up more in closely spaced plantings.

So in order to minimize the occurrence of albino fruit, especially during a stretch of overcast conditions following warm weather, one would want to be a little more reticent about nitrogen and water use.  Regardless, a change in the weather, i.e. from overcast conditions back to warm sunny weather such as we are experiencing today, is going to go miles in clearing up a spate of albino fruit.

Hat tip to the PCA for bringing this up with me.  It's always great to be in touch with the experts out in the field looking at this stuff day in and day out and figuring through these problems.

Situation: The complaint we were invited to evaluate in this field was less typical than what one often finds with yellow plants in Salinas or Watsonville. These patches of yellow plants were typically dispersed in patches of various sizes in the field (photo 1 below), but on one side of the field this was particularly pronounced.  This area corresponded with a farm road from a previous artichoke plantation as well as being the end of the drip tapes installed by the strawberry grower.

The bed tops tended to be dry and moisture has been adequate but not excessive, so my running thesis of excess water actually did not fit so well in this situation. 

I had the good fortune on this particular call of being accompanied by Frank Shields from Soil Control Lab, who did a thorough evaluation of a bed and plant tissue (photo 2 below) and arrived at the results as depicted in photos 3 and 4 below.

Evaluation: As readers can assess from the attached analyses, this sampling was extraordinarily detailed.  The soil analysis breaks down the bed into ten zones and evaluates no less than 14 parameters of each zone.  Furthermore, in zone 7, Frank took a general sample of the soil texture and shrinkage, which gives an assessment of at what moisture percentage cracks form in the soil.

As a standard operating procedure, Frank sampled one bed containing symptomatic plants according to a pattern of ten different areas, the cross section of which can be seen in the soil report attached below.  Additionally, Frank uprooted several plants for evaluation of the roots and tissue mineral contents in the foliage.

This soil test strives to accomplish a number of things.  First, it is designed to monitor the movement and location of accumulated soluble salts for the purpose of determining the water pattern from the irrigation system. Similarly, since during the season strawberry fertilizer is supplemented via the water it is useful to know where the nutrients are ending in the bed to confirm that they are getting to the plant and not being leached out of range or being affected by adverse pH or salt conditions.

Plant available nutrients are both water soluble and exchangeable. This analysis is designed to monitor the water soluble fraction for the water soluble plant nutrients nitrate, nitrite, chloride, and sulfate as well as cations which accumulate with the water soluble fraction like calcium, potassium, sodium and ammonia. 

Interpretation of Results:

Moisture: All zones were very wet indicating recent irrigation of sufficient volume to completely fill the bed and should leach salts and nutrients down and out of the bed.   Additionally, there were no shrinkage cracks visible when observing the fifty foot section of row again indicating that the soil had been kept sufficiently moist.

The pH is on the high side and evenly distributed between 7.8 and 8.4 though all zones.  However, this pH is not unusually high and normally would not be said to be problematic for strawberries.

Soil potassium is this sample low.   It is typical for soils in Salinas and the Pajaro Valley to have potassium concentrations above 150 ppm, but with the exception of zone 10 of the bed this particular sample ranges around 50 ppm or below.  This is reflected in the leaf potassium concentration of 0.9% when a range of 1.3 – 1.8% is ideal for this time of year.

Soil nitrate appears to be substantially leached out, and indeed the percentage in the leaves of the sampled yellow plants is 2.0%, which is somewhat less than the optimum of 2.4- 3.0 % for this time of year.   Still, 2.0 % dry weight tissue nitrogen would not explain the substantial yellowing we observe in the field.

Phosphorous, calcium, magnesium and the other micronutrients appear to be sufficient according to the attached tissue analysis.

Nitrites, which are oxidized to plant available nitrate in the process of nitrification but toxic to plants in quantity are absent from the sample.  This is quite probably because of the well aerated soil and lack of packing. 

EC5: This is a measure of the amount of water soluble components in a zone. Zone six has the highest in the root zones (1 – 8) but all within values to support plant growth. With proper watering, salts should accumulate in zone six, nine and ten. In this sample, salt accumulation is highest at zone ten typical of a pattern of a bed having full mulch and plenty of water.

We can also look at the ratio of water soluble cations to get an idea on the ratio of plant available constituents like the SAR value and soluble Ca/Mg ratio. The very high sodium and chloride compared to the low calcium and magnesium in this sample is significant and indicates a problem.   Indeed, sodium and chloride are excessively high in the plant tissue and very likely to be interfering with normal plant function.

Outside of the carbonates, the soil minerals are an accumulation both from the irrigation water and nutrients added to the soil. If most of the salts accumulated in the bed are naturally found in the irrigation water (as is the case here), it means that the nutrients are being leached out of the root zone with excess water and additional fertility could be considered.  

Conclusion:   Rather than being a problem of the deficiencies of nitrogen or potassium, Frank maintains that at issue here is a buildup in the affected areas of chloride and accumulated salts as a result of irrigation.  Carrying this thesis further, perhaps if the amount of current irrigation water could be limited in the areas experiencing yellow plants, one would also be reducing the amounts of apparently damaging sodium and chloride.  That might be one way to address the problem.

The scattershot pattern of yellow plants across the field is the major confounding point of the problem being evaluated here.  On the one hand it would indicate that the toxicities and deficiencies described above are occurring in the same scattershot pattern across the field but it does beg the question why the differences are so dramatic, sometimes even from one plant to the next.

So, while this evaluation has given us a good look at what is going on around these yellow plants, we still don't conclusively know what exactly is the cause of this problem.  It absolutely merits further work. 

Thank you to the grower who invited us out.  I thank Frank Shields of Soil Control Lab for the contribution of his time and expertise to working on this problem. 

Here is an interesting case regarding a slight purpling of the newer leaves of raspberry.  While the case below involves ‘Polka’ variety red raspberry, I’ve seen it this year on ‘Josephine’ red raspberry in a different field as well.

The question posed is whether this purpling is meaningful from a plant health standpoint.   Will this problem get worse and detract from yield and cause problems with next years crop?

For starters, there is are no disease symptoms, for example necrotic spots or goo seeping out out of the leaves or stems, nor are there any signs of disease, such as spores or conidial structures visible.

We should consider also possible side effects of insecticide or fungicide sprays.  I do recall once in a trial on the strawberry variety ‘Diamante’ that repeated applications of a strobilurin fungicide such as Pristine or Quadris resulted in a similar pattern of purpling on the leaves.  However in this case on ‘Polka’, the PCA in charge of this field confirmed with me that Pristine had been applied after the symptoms appeared, and this is only one application of the material.  There was only one other pesticide application previous to this one, and it was more than a month ago.

What about nutrient deficiency?  A simple application of what we know from nutrient deficiency books would inform us that the purpling we see here refers to some sort of phosphorous deficiency, but other nutrients can cause this too.  Furthermore, those having more than a passing knowledge of the agricultural soils in the Pajaro and Salinas valleys know they are rarely phosphorous deficient, and more often than not actually have an excess of this nutrient. 

Which brings us to nitrogen.  Nitrogen, while commonly associated with yellowing rather than purpling of leaves, leaches out of the soil easily and as such can be deficient even in the rich soils of the California central coast.   As we know, nitrogen deficiencies can be manifested in plants as a reddening or purpling of the leaves stemming from an accumulation of the same carbohydrates resulting from phosphorous deficiency.

The only way we are going to know if the above has any truth to it at all however is to take some leaf samples.  The chart below is from a bulk sample consisting of at least twenty leaves, each taken from around the fifth leaf of the plant (note that this is a bit younger than the seventh leaf common for sampling, but the purpling was only found at this stage and younger). 

Samples were analyzed by the Soil Control Lab in Watsonville. 

The chart above shows us that for one there are no dramatic differences between in nutrient concentrations of green leaves compared to those which are purple.  On the other hand, concentrations of nitrogen, phosphorous and potassium are trending just a tad low, with perhaps the nitrogen being the most significant especially since the sampled leaves were on the young side.